rspНаучно-практическая ревматологияRheumatology Science and Practice1995-44841995-4492IMA-PRESS, LLC10.14412/1995-4484-2003-1129rsp-1047Research ArticleСтатьиArticlesАнтикератиновые антитела при ревматоидном артритеAntikeratin antibodies in rheumatoid arthritisZabekJZabekJ-200315022003411№1 (2003)2728Copyright © Zabek J., 20032003Zabek J.Zabek J.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://rsp.mediar-press.net/rsp/article/view/1047

Цель. Определить частоту выявления антикератиновых антител (АКА) при РА. Материал и методы. АКА определялись методами непрямой иммунофлюоресцениии (НИФ) и Westem-blott в сыворотках 58 больных РА (43 жен и 15 муж) и 30 здоровых доноров. Результаты. АКА были обнаружены у 76% больных РА вне зависимости от длительности заболевания и возраста пациентов. Специфичность АКА для РА составила 96%. Заключение. Вопрос о роли АКА в развитии РА и их диагностическая ценность требуют дальнейшего изучения. Ключевые слова: антикератиновые антитела, ревматоидный артрит, диагностика.

Objective. To assess frequency of antikeratin antibodies (AKA) in rheumatoid arthritis (RA). Methods. AKA were measured by indirect immunofluorescence and Western blott in serum of 58 RA patients (43 f and 15 m) and 30 healthy donors. Results. AKA were found in 76% of RA patients not depending of duration of the disease and age of patients. AKA specificity for RA was 96%. Conclusion. Role of AKA in the development of RA and their diagnostic significance need further examination.

антикератиновые антителаревматоидный артритдиагностикаReferences<div><p>Aho К., Palusuo Т., Kurki P. Marker antibodies of rheumatoid arthritis: diagnostic and pathogenetic implications. Semin. Arthr. Rheum., 1994, 23, 379.</p><p>Berthelot J.M., Maugars Y., Castagne A. et al. Antiperinuclear factors are present in polyarthritis before ACR criteria for rheumatoid arthritis are fulfilled. Ann. Rheum. Dis., 1997, 56, 123.</p><p>Bizzaro N. Diagnostic accuracy of the anti-citrulline antibody assay for rheumatoid arthritis. Clin. Chemistry, 2001, 47, 1089.'</p><p>Cordonnier C., Meyer O., Palazzo E. et al. Diagnostic value of anti-RA33 antibody, antikeratin antibody, antiperinuclear factor and antinuclear antibody in early rheumatoid arthritis: comparison with rheumatoid factor. Br. J. Rheumatol., 1996, 35, 620.</p><p>Johnson G.D., Carvalho A., Holborow E.J. et al. Antiperinuclear factor and keratin antibodies in rheumatoid arthritis. Ann. Rheum. Dis., 1981, 40, 263.</p><p>Kirstein H., Mathiesen F.K. Antikeratin antibodies in rheumatoid arthritis. Scand. J. Rheumatol., 1987, 16, 331.</p><p>Kurki P., Aho K., Palosuo T. et al. Immunopathology of rheumatoid arthritis. Arthr.Rheum., 1992, 35, 8, 914.</p><p>Palosuo Т., Lukka М., Alenius H. et al. Purification of filaggrin from human epidermis and measurement of antifi- laggrin autoantibodies in sera from patients with rheumatoid arthritis by an enzyme-linked immunosorbent assay. Int. Arch. Allerg. Immunol., 1998, 115, 294.</p><p>Quismorio F.P., Kaufman R.L., Beardmore T. et al. Reactivity of serum antibodies to the keratin layer of rat esophagus in patients with rheumatoid arthritis. Arthr. Rheum., 1983, 26, 494.</p><p>Schellekens G.A. Citrulline is an essential constituent of antigens determinants recognized by rheumatoid arthritis - specific autoantibodies. J. Clin. Invest., 1998, 101, 273.</p><p>Serre G.: Autoantibodies to filaggrin/deiminated fibrin /AFA/ are useful for the diagnosis and prognosis of rheumatoid arthritis, and are probably involved in the pathophysiology of the disease. Joint Bone Spine, 2001, 68, 105.</p><p>Serre G. Vincent C.: Filaggrin /keratin/ autoantibodies. In: Peter J.B., Shoenfield Y. ed. Autoantibodies. Elsevier, Amsterdam, London, New York, 1996, 271.</p><p>Simon М., Girbal E., Sebbag M. et al. The cytokeratin filament-aggregating protein filaggrin is the target of the so- called "antikeratin antibodies”, autoantibodies specific for rheumatoid arthritis. J. Clin. Invest., 1993, 92, 1387.</p><p>Slack S.L. Mannik М., Dale B.A. Diagnostic value of antibodies to filaggrin in rheumatoid arthritis. J. Rheumatol., 1998, 25, 5, 847.</p><p>Smolen J.S.: Autoantibodies in rheumatoid arthritis. Autoantibody Manual CI.l., 1996, 1, 1.</p><p>Vincent Ch., Berthelot J.M., Youinou P. et al. The antigens defined by antikeratin antibodies /АКА/. Autoantibody Manual В 1.5, 1996, 1, 12.</p><p>Vincent Ch., Serre G., Lapeyre F. et al.: High diagnostic value in rheumatoid arthritis of antibodies to the stratum comeum of rat oesophagus epithelium, so-called "antikeratin antibodies". Ann. Rheum. Dis., 1989, 48, 712.</p><p>Young B.J.J., Mallaya R.K., Leslie R.D.J. et al. Antikeratin antibodies in rheumatoid arthritis. Br. Med. J., 1979, 2, 97.</p><p>Zabek J., Brzezicki J., Biemacka E. et al. Przeciwciala przeciwko cytokeratynie jako marker wczesnego reuma- toidalnego zapalenia stawow. Reumatol., 2001, 39, 303.</p></div><br /><div><p>Aho К., Palusuo Т., Kurki P. Marker antibodies of rheumatoid arthritis: diagnostic and pathogenetic implications. Semin. Arthr. Rheum., 1994, 23, 379.</p><p>Berthelot J.M., Maugars Y., Castagne A. et al. Antiperinuclear factors are present in polyarthritis before ACR criteria for rheumatoid arthritis are fulfilled. Ann. Rheum. Dis., 1997, 56, 123.</p><p>Bizzaro N. Diagnostic accuracy of the anti-citrulline antibody assay for rheumatoid arthritis. Clin. Chemistry, 2001, 47, 1089.'</p><p>Cordonnier C., Meyer O., Palazzo E. et al. Diagnostic value of anti-RA33 antibody, antikeratin antibody, antiperinuclear factor and antinuclear antibody in early rheumatoid arthritis: comparison with rheumatoid factor. Br. J. Rheumatol., 1996, 35, 620.</p><p>Johnson G.D., Carvalho A., Holborow E.J. et al. Antiperinuclear factor and keratin antibodies in rheumatoid arthritis. Ann. Rheum. Dis., 1981, 40, 263.</p><p>Kirstein H., Mathiesen F.K. Antikeratin antibodies in rheumatoid arthritis. Scand. J. Rheumatol., 1987, 16, 331.</p><p>Kurki P., Aho K., Palosuo T. et al. Immunopathology of rheumatoid arthritis. Arthr.Rheum., 1992, 35, 8, 914.</p><p>Palosuo Т., Lukka М., Alenius H. et al. Purification of filaggrin from human epidermis and measurement of antifi- laggrin autoantibodies in sera from patients with rheumatoid arthritis by an enzyme-linked immunosorbent assay. Int. Arch. Allerg. Immunol., 1998, 115, 294.</p><p>Quismorio F.P., Kaufman R.L., Beardmore T. et al. Reactivity of serum antibodies to the keratin layer of rat esophagus in patients with rheumatoid arthritis. Arthr. Rheum., 1983, 26, 494.</p><p>Schellekens G.A. Citrulline is an essential constituent of antigens determinants recognized by rheumatoid arthritis - specific autoantibodies. J. Clin. Invest., 1998, 101, 273.</p><p>Serre G.: Autoantibodies to filaggrin/deiminated fibrin /AFA/ are useful for the diagnosis and prognosis of rheumatoid arthritis, and are probably involved in the pathophysiology of the disease. Joint Bone Spine, 2001, 68, 105.</p><p>Serre G. Vincent C.: Filaggrin /keratin/ autoantibodies. In: Peter J.B., Shoenfield Y. ed. Autoantibodies. Elsevier, Amsterdam, London, New York, 1996, 271.</p><p>Simon М., Girbal E., Sebbag M. et al. The cytokeratin filament-aggregating protein filaggrin is the target of the so- called "antikeratin antibodies”, autoantibodies specific for rheumatoid arthritis. J. Clin. Invest., 1993, 92, 1387.</p><p>Slack S.L. Mannik М., Dale B.A. Diagnostic value of antibodies to filaggrin in rheumatoid arthritis. J. Rheumatol., 1998, 25, 5, 847.</p><p>Smolen J.S.: Autoantibodies in rheumatoid arthritis. Autoantibody Manual CI.l., 1996, 1, 1.</p><p>Vincent Ch., Berthelot J.M., Youinou P. et al. The antigens defined by antikeratin antibodies /АКА/. Autoantibody Manual В 1.5, 1996, 1, 12.</p><p>Vincent Ch., Serre G., Lapeyre F. et al.: High diagnostic value in rheumatoid arthritis of antibodies to the stratum comeum of rat oesophagus epithelium, so-called "antikeratin antibodies". Ann. Rheum. Dis., 1989, 48, 712.</p><p>Young B.J.J., Mallaya R.K., Leslie R.D.J. et al. Antikeratin antibodies in rheumatoid arthritis. Br. Med. J., 1979, 2, 97.</p><p>Zabek J., Brzezicki J., Biemacka E. et al. Przeciwciala przeciwko cytokeratynie jako marker wczesnego reuma- toidalnego zapalenia stawow. Reumatol., 2001, 39, 303.</p></div><br />

The authors declare that there are no conflicts of interest present.