rspНаучно-практическая ревматологияRheumatology Science and Practice1995-44841995-4492IMA-PRESS, LLC10.14412/1995-4484-2014-169-173rsp-1920Research ArticleОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯORIGINAL RESEARCHОценка содержания CD4+CD25+CD127low регуляторных Т-лимфоцитов и их функционального состояния по экспрессии молекул CTLA-4 и CD39 у больных ревматоидным артритомEVALUATION OF THE CONTENT OF CD4+CD25+CD127LOW REGULATORY T CELLS AND THEIR FUNCTIONAL STATUS ACCORDING TO THE EXPRESSION OF CTLA-4 AND CD39 MOLECULES IN PATIENTS WITH RHEUMATOID ARTHRITISЖулайГ. А.ZhulaiG. A.zhgali-111@rambler.ruОлейникЕ. К.OleinikE. K.БарышеваО. Ю.BaryshevaO. Yu.ЧуровА. В.ChurovA. V.ОлейникВ. М.OleinikV. M.КравченкоП. Н.KravchenkoP. N.КучинА. А.KuchinA. A.ФГБУН «Институт биологии Карельского научного центра» РАН, Петрозаводск, РоссияРоссияInstitute of Biology, Karelian Research Center of the Russian Academy of Sciences, Petrozavodsk, RussiaRussian FederationФГБОУ ВПО «Петрозаводский государственный университет» Петрозаводск, РоссияРоссияPetrozavodsk State University, Petrozavodsk, RussiaRussian Federation201417062014522НАУЧНО-ПРАКТИЧЕСКАЯ РЕВМАТОЛОГИЯ 52 No 2-2014169173Copyright © Жулай Г.А., Олейник Е.К., Барышева О.Ю., Чуров А.В., Олейник В.М., Кравченко П.Н., Кучин А.А., 20142014Жулай Г.А., Олейник Е.К., Барышева О.Ю., Чуров А.В., Олейник В.М., Кравченко П.Н., Кучин А.А.Zhulai G.A., Oleinik E.K., Barysheva O.Y., Churov A.V., Oleinik V.M., Kravchenko P.N., Kuchin A.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://rsp.mediar-press.net/rsp/article/view/1920

Цель исследования – изучить содержание супрессорной популяции регуляторных Т-клеток (Tper) по экс- прессии молекул CD4, CD25, CD127, а также уровень экспрессии ими двух функциональных молекул (CTLA-4 и CD39) у больных ревматоидным артритом (РА). Материал и методы. Исследованы 16 образцов периферической крови больных РА и 10 образцов крови здо- ровых доноров. Все больные получали терапию базисными противовоспалительными препаратами. Уровень экспрессии всех исследуемых молекул оценивался методом проточной цитофлюориметрии. Результаты исследования. Содержание Т-хелперов в периферической крови больных РА составило 36,3±7,1% от общего числа лимфоцитов и было ниже, чем в контроле (43,8±6,2%, р<0,05), а число активированных CD4+CD25+ Т-клеток у этих больных было увеличено в 2 раза (РА 23,7±9,8%, контроль 11,1±2,0% от числа CD4+ T-клеток; р<0,05). Относительное количество CD4+CD25high (РА 2,7±1,0%, контроль 1,5±0,8%) и CD4+CD25highCD127low/- (РА 2,5±1,0%, контроль 1,6±0,9% от CD4+ Т-клеток) Tper-клеток у больных РА было достоверно больше, чем у здоровых лиц (р<0,05). Экспрессия Tper-клетками больных РА негативной регуляторной молекулы CTLA-4 и эктонуклеотидазы CD39 не отличалась от контроля. Заключение. Проведенное исследование показало, что РА характеризуется повышенным содержанием Tper- клеток фенотипов CD4+CD25high и CD4+CD25highCD127low, но не CD4+CD25+CD127low, а также схожей с контролем экспрессией Tper-клетками функциональных молекул CTLA-4 и CD39. 

Objective. To study the content of the suppressor population of regulatory T cells (Treg) according to the expression of CD4, CD25, CD127 molecules, as well as the expression of two functional molecules (CTLA-4 and CD39) in patients with rheumatoid arthritis (RA). Material and methods. 16 samples of peripheral blood of RA patients and 10 blood samples of healthy donors were analyzed. All patients received disease-modifying anti-rheumatic drugs. The expression level of all studied molecules was assessed by flow cytofluorometry. Results. The content of T-helpers in peripheral blood of RA patients was 36.3 ± 7.1% of the total number of lympho- cytes and was lower than that in the control (43.8 ± 6.2%, p<0.05). The number of activated CD4+CD25+ T cells in these patients increased twofold (RA 23.7 ± 9.8%, control 11.1 ± 2.0% of the number of CD4+ T cells, p<0.05). The relative amount of CD4+CD25high (RA 2.7 ± 1.0%, control 1.5 ± 0.8%) and CD4+CD25highCD127low/- (RA 2.5 ± 1.0%, control 1.6 ± 0.9% of CD4+ T cells) Treg cells in RA patients was significantly higher than that in healthy individuals (p<0.05). The expression of the CTLA-4 negative regulatory molecule and CD39 ectonucleotidase by Treg cells of RA patients did not differ from the control. Conclusion. The study demonstrated that RA is characterized by an increased content of Treg cells of CD4+CD25high and CD4+CD25highCD127low phenotypes rather than CD4+CD25+CD127low, as well as by the control-like expression of CTLA-4 and CD39 functional molecules by Treg cells.

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The authors declare that there are no conflicts of interest present.