rspНаучно-практическая ревматологияRheumatology Science and Practice1995-44841995-4492IMA-PRESS, LLC10.14412/1995-4484-2017-187-191rsp-2362Research ArticleОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯORIGINAL RESEARCHКлинико-патогенетическое значение белков теплового шока с массой 70 и 27 кДа при остеоартритеTHE CLINICAL AND PATHOGENETIC SIGNIFICANCE OF 70- AND 27-kDa HEAT SHOCK PROTEINS IN OSTEOARTHRITISКабалыкМ. А.KabalykM. A.ФГБОУ ВО «Тихоокеанский государственный медицинский университет» Минздрава России, ВладивостокРоссияPacific State Medical University, Ministry of Health of RussiaRussian Federation201724042017552187191Copyright © Кабалык М.А., 20172017Кабалык М.А.Kabalyk M.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://rsp.mediar-press.net/rsp/article/view/2362

Цель исследования – установить особенности изменений уровней белков теплового шока (БТШ), хемокинов и маркера деградации коллагена в крови больных остеартритом (ОА) в зависимости от стадии заболевания. Материал и методы. В условиях ревматологического кабинета Владивостокской поликлиники №3 было обследовано 99 пациентов с ОА коленных суставов. Диагноз был верифицирован в соответствии с критериями Европейской антиревматической лиги (EULAR) 2010 г. В этой группе было 87 (88%) женщин и 12 (12%) мужчин, средний возраст больных составил 66,7±7,9 года, длительность заболевания – 5,9±4,0 года. В качестве контрольной группы в исследование были включены 21 практически здоровая женщина и 9 мужчин в среднем возрасте 59,6±8,3 года. Для определения концентраций искомых молекул в крови пациентов, включенных исследование, проводился иммуноферментный анализ. Использовали ELISA kits HSP70, HSP27 (SunLong Biotech Co. Ltd, КНР), CRTAP (cartilage associated protein – хрящ-ассоциированный белок, ХАБ), TNFα (tumor necrosis factor α – фактор некроза опухоли α, ФНОα), CXCL17 (хемокин класса CXC) (Cloud-Clone Corp., США). Результаты и обсуждение. Уровни БТШ27, БТШ70, их соотношение и ХАБ при ОА были статистически значимо ниже, чем в контрольной группе. Уровни ФНОα и CXCL17, наоборот, значимо превышали показатели контрольной группы. У больных ОА БТШ70 продемонстрировал обратную корреляционную связь с уровнями ХАБ и ФНОα. ХАБ статистически значимо коррелировал с ФНОα. ФНОα имел прямую связь с CXCL17. При длительности заболевания 10 лет и более уровень ХАБ был достоверно выше, чем у больных, у которых анамнез ОА составлял 5–9 лет (p<0,05), и не отличался от такового у пациентов с продолжительностью ОА 1–4 года (p<0,05). Уровень CXCL17 статистически значимо снижался по мере увеличения продолжительности заболевания (p<0,05). В группе пациентов с давностью ОА 1–4 года длительность заболевания имела прямую значимую корреляционную связь с уровнями ХАБ и ФНОα. При давности анамнеза 5–9 лет наблюдалась достоверная прямая связь продолжительности ОА с ХАБ и CXCL17. У заболевших 10 лет назад и более длительность болезни прямо коррелировала с ХАБ, ФНОα и CXCL17.

 

Objective

Objective: to establish the specific features of changes in the levels of heat shock proteins (HSP), chemokines, and a marker for collagen degradation in the blood of patients with osteoarthritis (OA) depending on the stage of the disease. Subjects and methods. 99 patients with knee OA were examined in the rheumatology room, Vladivostok Polyclinic Three. The diagnosis was verified in accordance with the 2010 European League Against Rheumatism (EULAR) criteria. This group comprised 87 (88%) women and 12 (12%) men; the patients’ mean age was 66.7±7.9 years; the disease duration was 5.9±4.0 years. A control group included 21 apparently healthy women and 9 men; their mean age was 59.6±8.3 years. Enzyme immunoassay was carried out to determine the concentrations of query molecules in the blood of the patients included in the investigation. The investigators used ELISA kits for HSP70, HSP27 (SunLong Biotech Co. Ltd, China), CRTAP (cartilage-associated protein, CAP), TNF-α (tumor necrosis factor-α), and CXCL17 (chemokine (C-X-C motif) ligand 17) (Cloud-Clone Corp., USA). Results and discussion. The levels of HSP27 and HSP70, their ratio, and CAP in OA were significantly lower than those in the control group. Those of TNF-α and CXCL17 were, on the contrary, considerably higher than those in the control group. In the patients with OA, HSP70 demonstrated an inverse correlation with the levels of CAP and TNF- α. CAP was statistically significantly correlated with TNF-α. The latter was directly related to CXCL17. When the duration of the disease was 10 or more years, the level of CAP was significantly higher than that in patients with a 5–9-year history of OA (p < 0.05) and did not differ from that in those with OA of 1–4-year duration (p > 0.05). The level of CXCL17 reduced statistically significantly with longer disease duration (p < 0.05). In a group of patients with a 1–4-year history of OA, the disease duration had a direct significant correlation with the levels of CAP and TNF-α. When the history of the disease was 5–9 years, there was a significant direct relationship of the duration of OA to CAP and CXCL17. In the patients who fell ill 10 or more years ago, the duration of the disease was directly correlated with the levels of CAP, TNF-α, and CXCL17.

 

 

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The authors declare that there are no conflicts of interest present.