rspНаучно-практическая ревматологияRheumatology Science and Practice1995-44841995-4492IMA-PRESS, LLC10.14412/1995-4484-2017-245-251rsp-2376Research ArticleОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯORIGINAL RESEARCHВзаимосвязь FoxP3+ регуляторных Т-клеток с активностью заболевания и уровнем антител при раннем ревматоидном артритеTHE RELATIONSHIP OF FoxP3+ T REGULATORY CELLS TO DISEASE ACTIVITY AND ANTIBODY LEVELS IN EARLY RHEUMATOID ARTHRITISАвдееваА. С.AvdeevaA. S.

115522 Москва, Каширское шоссе, 34А

34A, Kashirskoe Shosse, Moscow 115522

9056249400@mail.ruРубцовЮ. П.RubtsovYu. P.

кафедра биохимии и молекулярной медицины факультета фундаментальной медицины

119192 Москва, Ломоносовский проспект, 31, корп. 5

Department of Biochemistry and Molecular Medicine, Faculty of Fundamental Medicine

31, Lomonosovsky Prospect, Build. 5, Moscow 119192

ДыйкановД. Т.DyikanovD. T.

кафедра биохимии и молекулярной медицины факультета фундаментальной медицины

119192 Москва, Ломоносовский проспект, 31, корп. 5

Department of Biochemistry and Molecular Medicine, Faculty of Fundamental Medicine

31, Lomonosovsky Prospect, Build. 5, Moscow 119192

ПопковаТ. В.PopkovaT. V.

115522 Москва, Каширское шоссе, 34А

34A, Kashirskoe Shosse, Moscow 115522

НасоновЕ. Л.NasonovE. L.

кафедра ревматологии Института профессионального образования

115522 Москва, Каширское шоссе, 34А

119991 Москва, ул. Трубецкая, 8, стр. 2 

Department of Rheumatology, Institute of Professional Education

34A, Kashirskoe Shosse, Moscow 115522

8, Trubetskaya St., Build. 2, Moscow 119991 

ФГБНУ «Научно-исследовательский институт ревматологии им. В.А. Насоновой», МоскваРоссияV.A. Nasonova Research Institute of Rheumatology, MoscowRussian FederationФГБОУ ВО «Московский государственный университет им. М.В. Ломоносова»РоссияM.V. Lomonosov Moscow State UniversityRussian FederationФГБНУ «Научно-исследовательский институт ревматологии им. В.А. Насоновой», Москва; ФГАОУ ВО «Первый Московский государственный медицинский университет им. И.М. Сеченова» Минздрава РоссииРоссияV.A. Nasonova Research Institute of Rheumatology, Moscow; I.M. Sechenov First Moscow State Medical University, Ministry of Health of Russia, MoscowRussian Federation201709072017553245251Copyright © Авдеева А.С., Рубцов Ю.П., Дыйканов Д.Т., Попкова Т.В., Насонов Е.Л., 20172017Авдеева А.С., Рубцов Ю.П., Дыйканов Д.Т., Попкова Т.В., Насонов Е.Л.Avdeeva A.S., Rubtsov Y.P., Dyikanov D.T., Popkova T.V., Nasonov E.L.This work is licensed under a Creative Commons Attribution 4.0 License.https://rsp.mediar-press.net/rsp/article/view/2376

Цель – проанализировать взаимосвязь количества FoxP3+ регуляторных Т-клеток (Трег) с клинико-лабораторными показателями активности заболевания и уровнем антител в группе пациентов с ранним ревматоидным артритом (РА).

Материал и методы

Материал и методы. В исследование были включены 45 не получавших ранее терапии метотрексатом пациентов с ранним РА (критерии ACR/EULAR 2010 г.), в том числе 39 женщин; медиана возраста составила 52,0 [32,5; 57,5] года, длительность заболевания – 5 [4; 6] мес, DAS28 – 5,01 [4,18; 5,8]; 71,1% больных были позитивны по ревматоидному фактору (РФ) и 88,9% позитивны по антителам к циклическому цитруллинированному пептиду (АЦЦП). Относительное и абсолютное количество Трег (FoxP3+CD25+; CD152+surface; CD152+intracellular; FoxP3+CD127-; CD25+CD127-; FoxP3+ICOS+; FoxP3+CD154+; FoxP3+CD274+) определялось методом иммунофлюоресцентного окрашивания и многоцветной проточной цитофлюориметрии. Контрольную группу составили 20 здоровых доноров, сопоставимых по полу и возрасту с обследованными больными.

Результаты и обсуждение

Результаты и обсуждение. У 22 (48,9%) больных была высокая, у 20 (44,4%) – умеренная и у 3 (6,7%) – низкая активность патологического процесса по DАS28. У пациентов с ранним РА, по сравнению со здоровыми донорами, отмечалось более низкое процентное количество (ПК) FoxP3+CD25+ клеток, ПК и абсолютное содержание (абс.) FoxP3+ICOS+ клеток, ПК и абс. FoxP3+CD154+ и FoxP3+ CD274+ Т-клеток; p<0,05 во всех случаях. Регистрировалась отрицательная корреляционная взаимосвязь: ПК FoxP3+CD25+ с С-реактивным белком (СРБ) (r=-0,4); ПК CD152+intracellular с DAS28 (r=-0,35), СОЭ (r=-0,46), СРБ (r=-0,54); ПК FoxP3+CD127- с СРБ (r= -0,42); ПК CD25+CD127- с DAS28 (r=-0,38), SDAI (r=-0,41), CDAI (r=-0,36), СОЭ (r=-0,39), СРБ (r=-0,47); р<0,05 во всех случаях.

Среди серонегативных по РФ больных была выявлена более высокая ПК CD25+CD127-, ПК и абс. Foxp3+CD154+ и Foxp3+CD274+ Т-лимфоцитов.

Заключение

Заключение. Представленные данные позволяют говорить о снижении количества и функциональной активности Трег при раннем РА, что ассоциируется с более высокой активностью заболевания, наличием системных проявлений болезни, а также сопровождается гиперпродукцией антител.

Objective

Objective: to analyze the relationship of the count of FoxP3+ T regulatory cells (Tregs) to the clinical and laboratory parameters of disease activity and the levels of antibodies in a group of patients with early rheumatoid arthritis (RA).

Subjects and methods

Subjects and methods. The investigation enrolled 45 patients with early RA (2010 ACR/EULAR criteria) who had not previously received treatment with methotrexate, including 39 women; median age was 52.0 [32.5; 57.5] years; disease duration, 5 [4; 6] months, DAS28 5.01 [4.18; 5.8]; 71.1% of the patients were rheumatoid factor (RF) positive and 88.9% were anti-cyclic citrullinated peptide positive. The relative and absolute counts of Treg (FoxP3+CD25+; CD152+surface; CD152+intracellular; FoxP3+CD127-; CD25+CD127-; FoxP3+ICOS+; FoxP3+CD154+; FoxP3+CD274+) were measured by immunofluorescence staining and multicolor flow cytometry. A control group consisted of 20 healthy donors who were matched for sex and age with the examined patients.

Results and discussion

Results and discussion. DАS28 was high, moderate, and low in 22 (48.9%), 20 (44.4%), and 3 (6.7%) patients, respectively. As compared with the healthy donors, the patients with early RA were observed to have lower values in the percentage of FoxP3+CD25+ cells, in the percentage and absolute count of FoxP3+ICOS+ cells, in the percentage and absolute count of FoxP3+CD154+ and FoxP3+ CD274+ T cells; p<0.05 in all cases. Negative correlation was recorded between the percentage of FoxP3+CD25+ and C-reactive protein (CRP) (r=-0.4); that of CD152+intracellular and DAS28 (r=-0.35), ESR (r=-0.46), CRP (r=-0.54); that of FoxP3+CD127 and CRP (r=-0.42); that of CD25+CD127 and DAS28 (r=-0.38), SDAI (r=-0.41), CDAI (r=-0.36), ESR (r=-0.39), CRP (r=-0.47); p<0.05 in all cases.

The patients who were seronegative for RF were found to have higher values in the percentage of CD25+CD127, in the percentage and absolute count of Foxp3+CD154+ and Foxp3+CD274+ T lymphocytes.

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The authors declare that there are no conflicts of interest present.