Особенности фенотипа Т-регуляторных клеток при раннем ревматоидном артрите

Цель – изучить содержание и особенности фенотипа Т-регуляторных клеток (Трег) в периферической крови здоровых доноров и пациентов с ранним ревматоидным артритом (РА) методом многоцветной проточной цитометрии.

Материал и методы. В исследование было включено 39 пациентов с ранним РА. Процентное количество (ПК) и абсолютное содержание Трег (FoxP3+CD25+; CD152+surface; CD152+intracellular; FoxP3+CD127; CD25+CD127-; FoxP3+ICOS+; FoxP3+CD154+; FoxP3+CD274+) определялось методом многоцветной проточной цитофлуориметрии. Контрольную группу составили 20 здоровых доноров, сопоставимых по полу и возрасту с обследованными пациентами.

Результаты и обсуждение. Среди включенных в исследование больных медиана [25-й; 75-й перцентили] DAS28 составили 5,01 [4,2; 5,8], при этом у 22 (48,9%) регистрировалась высокая, у 20 (44,4%) – умеренная и у 3 (6,7%) – низкая активность патологического процесса. У пациентов с ранним РА по сравнению со здоровыми донорами регистрировались более низкое ПК FoxP3+CD25+ клеток, ПК и абсолютное содержание FoxP3+ICOS+, FoxP3+CD154+ и FoxP3+ CD274+ Т-клеток (p<0,05 во всех случаях). Регистрировалась отрицательная корреляционная взаимосвязь: ПК FoxP3+CD25+ с СРБ (r=-0,4); ПК CD152+intracellular с DAS28 (r=-0,35), СОЭ (r=-0,46) и СРБ (r=-0,54); ПК FoxP3+CD127- с СРБ (r=-0,42); ПК CD25+CD127- с DAS28 (r=-0,38), SDAI (r=-0,41), CDAI (r=-0,36), СОЭ (r=-0,39), СРБ (r=-0,47); р<0,05 во всех случаях.

Заключение. Полученные данные свидетельствуют о нарушении функциональной активности Трег при раннем РА, что оказывает влияние на активность воспалительного процесса.

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