Взаимосвязь содержания Трегуляторных клеток, активности и уровня сывороточных аутоантителу больных системной красной волчанкой на фоне ритуксимабом
https://doi.org/10.14412/1995-4484-2009-452
Аннотация
Цель. Выявить изменения количества CD+CD25+FoxP3+ T регуляторных клеток (Т рег) у больных СКВ, получающих ритуксимаб, и определить взаимосвязь содержа- ния Т рег с изменением активности заболевания и уровнем сывороточных аутоантител на фоне лечения. Материал и методы. Включены 12 пациенток с доставерной СКВ. ср. возраст больных 30,5±9,8 лет (Me – 31 лет, диапазон от 18 до 49 лет), продолжительность болезни 7-148 мес. Больные обследовались до начала лечения, через 1,3, 6 мес. Активность СКВ оценивали с помощью шкалы SLEDAI 2К. Помимо рутинных лабораторныхисследований определялись антитела к двухспиральной ДНК, количество В лимфоцитов и Трег клеток методом флоуцитометрии. Ритуксимаб вводился еженедельно внутривенно капельно по 500 мг в течение 4-х недель, у 4 больных проведена комбинированная терапия с циклофосфаном. Результаты. Количество Т-рег до начала терапии не было снижено. Не обнаружено корреляции между количеством Т-рег клеток и уровнем антител к ДНК. Корреляционный анализ показал значимую связь динамики маркеров Т-рег (Foxp3+, CD25+, CD4+CD25+), а также значения активности СКВ по SLEDAI 2K после завершения лечения с наличием клинического ответа на терапию ритуксимабом через 3 мес. Модель бинарной логистической регрессии с включением указанных 4 предикторов в совокупности способна корректно предсказать клинический ответ на терапию в 83% случаев. Заключение. Изменение количества СD25+, CD4+CD25+, СD4+CD25+FoxP3+ T клеток сразу после окончания лечения коррелировало с клиническим эффектом терапии ритуксимабом и определяло последующий ответ на анти-В-клеточную терапию больных СКВ.
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Рецензия
Для цитирования:
Torgashina A.V. Взаимосвязь содержания Трегуляторных клеток, активности и уровня сывороточных аутоантителу больных системной красной волчанкой на фоне ритуксимабом. Научно-практическая ревматология. 2009;47(2):3-9. https://doi.org/10.14412/1995-4484-2009-452
For citation:
Torgashina A.V. Relationship of T regulatory cells content, activity and serum autoantibodies level in patients with systemic lupus erythematosus receiving rituximab. Rheumatology Science and Practice. 2009;47(2):3-9. (In Russ.) https://doi.org/10.14412/1995-4484-2009-452
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